Both teams currently sit on three points and will face each other for the very first time in the Women's Super League, upon Tottenham's recent promotion to the competition.
West Ham looks to build the same success they had last year when Matt Beard's squad reached the FA Cup Final.
Irons' boss will almost have a fully-fit squad available to choose from.
Esmee de Graaf and Julia Simic are the only ones who will miss out this weekend's match due to recovering from their respective long-term injuries.
Alisha Lehmann made her first appearance of the season under the Hammers last Sunday in the FA Women's Continental League Cup and could be an option to feature Sunday. While Leanne Kiernan could also make the starting team after she earned her first competitive minutes in the same match last weekend.
Hotspur's Head Coach, Juan Amoros did not disclose any injuries on Spurs' official website but stated that the team is confident for the challenge.
“The girls are really buzzing to play, not even just in front of a big crowd, but to play another game. They are all eager to compete, we have a squad of players who all want to be on the pitch – every single player wants to be involved in an occasion like the one coming up this weekend.
“I know the girls are ready for it, they’re all looking forward to it and if we can go there and get three points and bounce back before the international break, it will be fantastic.”
The last time Tottenham visited West Ham in a league match dates back to May 2017, when Karen Hills' side achieved an impressive 7-0 win in what was the third-tier encounter.
A record to overcome:
The London club's boss has never won a Women's Super League London derby before. His previous encounters have ended in a draw and losing eight of his previous nine games across spells with Chelsea and West Ham.
A disappointing start to the campaign for both teams:
West Ham form:
West Ham has had a weak start to the season with only a win thus far, having failed to overcome teams in the top-flight as they lost five of their last six matches. Should they come away with the three points on Sunday, they will be looking to continue with their back-to-back wins following their 1-0 victory over Birmingham City last time out.
Spurs have won one and lost the other in the Barclays FA Women's Super League games so far this season but achieved their first-ever victory in the competition against Liverpool. Rachel Furness' penalty secured the team the three points.
Where and when?
The match will take place at the London Stadium on Sunday, September 29, followed by a 2:30 pm kick-off. It will also be available to watch live on BT Sport.