The Blades restarted their campaign with no wins in their opening four games before going unbeaten in their previous four.
Last weekend, Chris Wilder's men defeated Chelsea 3-0 at Bramall Lane and increased their chances of European football.
Thursday will offer a familiar test to the team in red and white, with the Foxes desperate to resume to winning ways in the final three games.
Back in 2005, Craig Levein and his Leicester side defeated the promotion chasers at the Walkers Stadium.
Perfect opening 15
Neil Warnock's side travelled to the East Midlands on an eight-game unbeaten run, whilst the Foxes had failed to win in their last four.
However, despite their fine form, United fell two goals behind courtesy of Iain Hume and Ryan Smith's neat finishes.
The first came after Hume managed to muscle off the defenders before latching onto Smith's cross.
Three minutes later, Smith headed past Paddy Kenny to double the Foxes lead.
Early in the second-half, Steven Kabba halved the deficit. However, Elvis Hammond restored the home side's two-goal lead with his second league goal of the campaign.
Hume made sure of the three points when he beat Kenny again, with another neat slotted finish past the goalkeeper.
The Blades netted a consolation goal in the 90th minute through Danny Webber. But the defeat meant Reading overtook United at the top of the Championship.