The first trophy for the 2020/21 season is up for grabs and Spurs will be desperate to progress, aiming to win their first piece of major silverware since 2008 - the year they last won this very competition.
Manager Jose Mourinho played a strong side in the quarter-final victory over Stoke City and is expected to field a similar side in a competition he has shown great importance over his years.
Brentford boss Thomas Frank has an almost fully fit squad to choose from, besides the injured Shandon Baptiste.
With their last game being 7 days prior to this fixture, Frank will have had enough time to allow his players to rest and recover, it may mean he sticks with the side that beat a good Bournemouth side.
Ones to watch
Tottenham: Dele Alli
Spurs midfielder Dele Alli has endured a tough time to the start of this campaign, resultantly, has been linked heavily with a move away in the January Transfer window.
He started the quarter-final against Stoke City and performed well however was brought off midway through the second-half after losing the ball and conceding as a result.
If given the opportunity to return to the starting XI, he will undoubtedly want to impress Mourinho and attempt to force his way back into his manager's plans from the fringes.
With Tottenham likely to field a strong side, there will be enough quality around Dele to make things happen, whether that be creating chances or scoring them.
Brentford: Ivan Toney
Former toon Ivan Toney will be Brentford's biggest threat heading into the clash against Spurs, he currently tops the scoring chart with 16 goals in the Championship in 22 games.
He has also provided 4 assists this campaign, with an impressive 32% shot conversion rate, Toney will have to be kept quiet by the Tottenham defence.
One of his main features is running in behind, constantly on the move, looking to get into the channels to exploit space and cause the Spurs backline problems.
He had been noticed by Tottenham scout David Pleat, prior to his move to Brentford and it will be interesting to see how he does against the North London club.
Tottenham (4-2-3-1): Lloris; Aurier, Sanchez, Dier, Davies; Hojbjerg, Sissoko; Moura, Alli, Son; Kane
Brentford (4-3-3): Raya; Dalsgaard, Jansson, Pinnock, Henry; Dasilva, Janelt, Jensen; Mbuemo, Toney, Canos
How to watch
Tottenham Hotspur vs Brentford will be broadcast live from the Tottenham Hotspur Stadium on Sky Sports Main Event and Sky Sport Football, kick-off will be at 7:45pm GMT.