The sides have met before in this competition, as then seventh tier Chorley beat Fourth Division Wolves 3-0 at Bolton's Burnden Park.
With Wolves facing a potential banana skin while on a poor run of form, Nuno is expected to put out a strong team at Victory Park.
The Portuguese has no new injury troubles to contend with following Saturday's Black Country derby, although Willy Boly may not be risked having come back from a hamstring injury last week.
For Chorley, former AFC Telford man Andy Owens could start alongside top-scorer Connor Hall after the 31-year-old scored in a 3-2 win over Farsley Celtic last weekend.
Chorley (4-1-3-2): Urwin; Birch, Leather, Halls, Baines; Tomlinson; Newby, Calveley, Shenton; Owens, Hall.
Wolves (3-4-3): Ruddy; Saiss, Coady, Kilman; Hoever, Neves, Vitinha, Ait-Nouri; Traore, Cutrone, Neto.
Ones to watch
Chorley's Hall has already made headlines by scoring in all three of the Magpies' ties against EFL sides, but will be looking to go one further against Premier League opposition.
Former Blackburn man Willem Tomlinson made his professional debut in the FA Cup fifth round against Manchester United, and has impressed since signing for Chorley in November.
For Wolves, Cutrone has another chance to impress before Nuno decides whether to loan the Italian out ahead of next week's deadline, while John Ruddy will look to prolong his Wolves career in what looks to be his final season at the club.
Unusually for a game between a sixth-tier and a top-flight side, Chorley and Wolves have met before in a game that still sends shivers down Wanderers' fans spines.
After a 1-1 draw at Molineux and a drawn replay at Burnden Park, fourth-tier, near bankrupt Wolves headed to Bolton for a second replay against the Magpies, with new signings Steve Bull and Andy Thompson watching on.
A brace from Charlie Cooper and a Mark Edwards goal saw the Northern Premier League side through to the second round at the third opportunity, while the defeat is widely considered to be the lowest point in Wolverhampton Wanderers' 144-year history.
How to watch
The game kicks off at 7.45 on Friday and is live on BT Sport.