The Brewers are without striker Kane Hemmings who sustained a hamstring injury and is now out for three weeks. He is not the only serious injury Hasselbaink must deal with, new signing Louis Moult is out for up to five months with an ankle injury.
Jacob Maddox was also replaced in the 38th minute of the Shrewsbury tie through an injury, however, it hasn't be revealed how long he will be out for.
Oxford made seven new signings in the summer with six of them making appearance in their first league game. Gavin Whyte, Ryan Williams, Steve Seddon, Jordan Thorniley all started whilst Marcus McGuane and Billy Bodin were introduced from the bench.
Nathan Holland was the only new signing to miss out. Two players missed the first league game to isolation but their identity was kept anonymous. Derick Osei Yaw missed the tie with an hamstring injury and is unlikley to return.
Burton Albion: Garratt, Brayford, Shaughnessy, Leak, Hamer, O'Connor, Oshilaja, Borthwick-Jackson, Powell, Smith, Akins
Oxford United: Stevens, Forde, Moore, Thorniley, Seddon, Brannagan, Henry, Williams, McGuane, Whyte, Bodin
Ones to watch
Jonny Smith grabbed an assist against Shrewsbury and could be an influential member of the team going forward this season. Last season he scored two and assisted four in 18 in which was his first season in Staffordshire. Under Hasselbaink he may be utilised more this season and at 24 has still plenty of time to grow and reach his potential.
Smith is predominantly a right midfielder but can play on the left. He has also featured through the centre and with two strikers, Hemmings and Moult and an attacking midfielder, Maddox all injured. It may give Smith a good chance to play in the cup either centrally or out wide. Despite not getting the man of the match award in the first league game he did impress and looked dangerous down the wing, with impressive deliveries.
Steve Seddon joined Oxford from Birmingham City after a loan spell at AFC Wimbledon and on his competitive debut scored from left-back. He boasted a 70% pass success rate, won five successful duels and four aerial duels. He also had two shots on target with one of them hitting the back of the net.
Seddon can play either in midfield or defence on the left side and has occasionally turned out in central midfield. In his 94 career appearances he has played 49 times at left-back with the rest being on the left side of midfield or across the midfield line. He has scored nine times and assisted 16 times showing his ability to lead an attack from deep and progress further up the field. This is dangerous for any opposition and could be crucial for Oxford in the cup tie.
This doesn't make good reading for Burton, in their last three meetings Oxford have won two with the other a draw. It gets worse for Burton though as those two losses were 4-0 and 5-1 in Oxford's favour. However, since 2010 the clubs have faced off 17 times with Burton winning six, Oxford winning five and six ending all square.
It looks like a fixture that oozes goals with 48 goals being scored in those 17 games.
How to watch
The game will be available to watch on the streaming platform IFollow and tickets are still available for the 19:45 BST kick-off. For all fans outside the UK it will also be available via IFollow.