Sunderland Ladies have been drawn away at Yeovil Town after entering the FA Women's Cup at the fifth round.
The Lady Black Cats know Yeovil well, having faced them in WSL 2 before their promotion to the top flight, and will be confident of overcoming an opponent who are in the division below.
However, the Lady Glovers have the benefit of match fitness at a competitive level, having entered the competition in the third round, whereas this will be Sunderland's first competitive fixture of 2016.
The hosts of this tie beat third-tier Bradford City 2-0 in the third round, before eliminating fellow WSL 2 side Bristol City on penalties at the weekend.
Fairweather aware of threat
Despite being a division below, Yeovil will be a difficult opponent for the Wearsiders - arguably the most difficult they could have drawn outside the top flight.
The Lady Glovers are one of the front runners for promotion to WSL 1 ahead of the new season and Sunderland manager Carlton Fairweather is well aware of the danger they pose.
Speaking to the club's official website, Fairweather described the tie as a "tough" one in which his side will have to be "in good form" to win.
Sunderland did do the double over the South West side in 2014, but the wins were not easy and instead very hard fought - producing 2-0 and 2-1 scorelines.
Fairweather recognises that Yeovil are not "an easy team to break down," but says his team will head into the tie confident and "look[ing] to go through to the next round."
The rest of Sunderland's WSL 1 rivals will also make their bows in this round as they bid to reach the quarter-finals.
The Lady Black Cats are one of three teams in the top tier to face teams in the second division, with the others being Reading - who will play Millwall Lionesses - and last season's runners-up Notts County - who face Sunderland's local rivals, Durham.
There are three all top flight ties, with Birmingham City hosting Arsenal, Doncaster Belles welcoming holders Chelsea and Manchester City travelling to Liverpool, as well as an all second division clash between Aston Villa and Everton.
The FA Women's Cup will also have a third tier team in the last eight this season, as Brighton & Hove Albion and Sporting Club Albion - both of the FA Women's Premier League Southern Division - were drawn against each other.