Hector Bellerin has been brought back into the final squad after initially missing out, with an injury to Real Madrid's Dani Carvajal opening the door for him to make the trip.
However, there is no Diego Costa, Juan Mata or Santi Cazorla, with only five other English-based players joining Bellerin for the tournament.
Key figures from the Euro 2012 victory such as Fernando Torres, Javi Martinez and Xavi Hernandez also miss out after clearly not impressing manager Vicente del Bosque sufficiently to make the final cut.
The full squad
Goalkeepers: David de Gea, Iker Casillas, Sergio Rico.
Defenders: Cesar Azpilicueta, Hector Bellerin, Marc Bartra, Jordi Alba, Juanfran, Gerard Pique, Sergio Ramos, Mikel San Jose.
Midfielders: Bruno Soriano, Sergio Busquets, Cesc Fabregas, Andres Iniesta, Koke, Thiago Alcantara, David Silva.
Forwards: Aritz Aduriz, Lucas Vazquez, Alvaro Morata, Nolito, Pedro.
Spanish giants fail to dominate
Perhaps surprisingly, only nine of the final 23-man roster play their club football for one of the Spanish top three of Barcelona, Real Madrid or Atletico Madrid.
Players such as Alvaro Morata, Thiago Alcantara and Iker Casillas have represented these sides in the past, but there is an even mix in the squad with players such as Aritz Aduriz given the chance to shine after impressive domestic seasons.
The 35-year-old striker plundered 35 goals in all competitions for Athletic Bilbao this season including 20 in La Liga, though he only has five full Spanish caps to his name at this point.