The Briton finished a collossal 24 points ahead of American Sarah Hammer and Jolien D'Hoore of Belgium.
Trott once again makes history, this time becoming the first British female to win four Olympic gold medals.
Team GB now have 18 golds so far in Rio.
Trott led the field overnight
With a commanding lead from the first three events overnight, the world champion from London in March was poised to add a fourth Olympic title to her collection.
Coming second in the opening scratch race on Monday, the Hertfordshire sprint- cyclist won both the individual pursuit and elimination races, to lie in a strong position going into the second day. Trott led D'Hoore by eight points, with rival Hammer a further two back.
In the knowledge that Trott won three of the six races of the event in London 2012, the 24 year-old was in arguably an even stronger position than her home games of four years ago.
Briton extends lead in time trial and flying lap
In Tuesday's earlier fourth event, Laura Trott extended her overall lead.
Annette Edmondson of Australia was fastest over the 500m race ahead of Trott, but nevertheless the Briton increased her lead over D'Hoore by another four points, going into the flying lap and crucial points race.
As part of the women's team pursuit that won gold on Saturday in the Velodrome, the superior power of the Brit should be telling and so it proved.
As the last to go in the penultimate event, Trott clocked a fastest time of 13.878 ahead of Edmondson to win her third event. She added 40 points to her tally with D'Hoore and Hammer only fourth and sixth respectively and going into the final race she led by an almost unassailable 24 points.
Points race victory parade
With ten sprints over 100 laps of the track classification in each finish was vital. Trott added eight points over the opening five sprints, leaving the chasing pack needing to lap the field for any chance of overhauling the dominant world champion.
Leading by 28 points, Trott just had to stay on her bike. A sixth sprint-win came and with her nearest rivals watching each other for the minor medals, coasted around the remaining laps to win another Olympic gold.