Both sides finished with a hard-fought point despite a cagey first half with both sides failing to create clear-cut chances and maintaining their defensive shapes.
Just before half-time, Reading midfielder Jess Fishlock came close to breaking the deadlock as she fired an effort against the woodwork.
Kelly Chambers's side continued where they left off, and centre-forward Amalie Eikeland fired a low effort that went agonisingly wide.
Midway through the second half, Tottenham striker Rosella Ayane glided a low cross down the right that found Angela Addison at the near post, but her effort went over the bar.
Moments later, Ayane fired a powerful shot straight at Grace Moloney and failed to convert the rebound.
In the closing stages, Kit Graham executed a dangerous cross from the right, with Alanna Kennedy's header forcing Moloney to produce an outstanding save.
Kelly Chambers' thoughts
After an eighth league draw of the season, Reading remain sixth in the table.
“I am happy, a clean sheet. For me, that’s the most important thing today. Disappointed not to take all three points especially in terms of the control we had, probably for the majority of the game,” said Chambers.
“Tottenham had a spell in the last 15 minutes of the game. For me, it was important to bounce back from Monday’s game and we haven’t been good enough in either box this season.
“I can make sure we can defend in our box better and hopefully we can do better higher up as we keep building that.”
Rehanne Skinner's thoughts
Tottenham head coach, Rehanne Skinner, was impressed by her side’s performance.
Rosella Ayane could have been on the scoresheet on a few occasions as Spurs ended their four-game losing streak.
“I thought we played really well. I think taking a point and a clean sheet away from home against a good competitive side like Reading," said Skinner.
“We were managing our shape in the first half and making sure that we were secure defensively. We made some changes in terms of the squad going into the second half and they were game-changers – pressed the game more effectively, created more chances and kept the pressure right until the final whistle.
“The performances are continuing to get better and as a collective, we just keep believing on what we are doing and then we create more chances as we have today.”