Having not won in their last five games, Rovers' top three chances have taken a huge blow.
Walsall come into this game having won last time out, the first victory for the Saddlers since Brian Dutton took over from Darrell Clarke. They will be looking to secure their League Two status in the remaining games of the season.
Caolan Lavery appears to be the only doubt for the hosts.
Tranmere are without top-scorer James Vaughan as he remains sidelined with a knee injury.
Scott Davies is also unavailable with a long term achilles injury and Jay Spearing remains a doubt after he was subbed off injured against Cambridge United.
Walsall: Rose, White, Clarke, Sadler, Melbourne, Kinsella, Perry, Osadebe, Wright, Gordon, Holden.
Tranmere Rovers: Murphy, Khan, Clarke, Monthe, MacDonald, O'Connor, Lewis, Crawford, Feeney, Lloyd-McGoldrick, Woolery.
Ones to watch
Holden was the stand-out player in Saturday's victory. His delivery, movement and hard work was key to the Saddlers securing three points. Should he keep fit and maintain this form, the end of the season could be soothing for the West Midlands club after a disappointing season.
The forward has eight league goals to his name this season, although he has not scored in his last three matches. As key striker Vaughan remains sidelined, now is the time for Woolery to step up. With six games remaining and Rovers sitting two points outside the top three, Woolery will have to deliver for his side if they are to gain promotion to the third tier without the trouble of the play-offs.
How to watch
Due to current restrictions in the UK, there will be no fans in attendance at Banks's Stadium. However, the game is available to watch on both club's iFollow platforms. Kick-off is at 19:00 BST on Tuesday April 13.