Micky Mellon's side currently sit in ninth position, only ajar from the play-offs on goal difference alongside three other teams.
With four wins from their previous five, Tranmere look difficult to breakdown right now, especially as Rovers currently lead the league in clean sheets.
The travelling U's on the other hand currently rest in 18th position, with their last league victory coming nearly a month ago.
Colchester have currently created the least amount of big chances in the entire division, which stems to the route of their problems in a lack of scoring.
Mellon will have defender Calum MacDonald back available after serving his suspension following his dismissal against Forest Green Rovers.
Lee O'Connor will be absent due to international duty, but Mellon confirmed there are no fresh injury concerns ahead of Friday.
Tommy Smith and Armando Dobra will both also be missing, representing their countries in the upcoming round of fixtures.
Tranmere Rovers: Doohan; Cogley, Davies, Clarke, Merrie, McManaman, Foley, Spearing, Morris, Hawkes, Nevitt
Colchester United: George; Coxe, Chambers, Eastman, Daniels, Skuse, Judge, Sears, Sarpong-Wiredu, Chilvers, Nouble
Ones to watch
A tremendously versatile midfielder who is proven in defensive, winger and attacking roles, Morris is a prime player in this Tranmere team.
With two goals and two assists already this campaign, Morris averages a rating of 7.08. The 27-year old is fast revealing himself as a fan favourite, having made over 100 appearances for the club and establishing himself as one of the first names on the team sheet.
It might take a while for Colchester fans to accept a former Ipswich Town stalwart, but Sears is making it much easier for them.
With three goals and an assist already this campaign, Sears can act on either wing, behind the striker or in the primary forward role. With his darting pace and powerful strike, Sears will look to bolster the Colchester attacking stats this season, and to blemish Tranmere's clean sheet lead.
Referee: James Oldham
Assistant referees: Steve Rushton and Anthony Moore
Fourth official: Paul Graham
These two sides failed to defeat one another last season, drawing 2-2 and 0-0 in the space on two months.
Ignoring the goalless draw, last time out it was a much more entertaining fixture after goals from Harry Pell and Brendan Sarpong-Wiredu gave Colchester a two goa lead. However, goals from Danny Lloyd and Jay Spearing masterminded an inspiring comeback from Tranmere.
This game is a 19:45 BST kick-off, but unfortunately cannot be watched in the UK. However, it can be watched internationally through the clubs' streaming services on their website. Tickets are also still available for the match, however pay on the gate is not permitted due to COVID-19 restrictions.