Gillingham are winless in their last five league contests and drew 1-1 with Oxford United over the weekend, the third time in the last four matches they have had a result with that scoreline.
Wes Hoolahan is nearing a recovery for Cambridge, but he may not be fit enough to feature on Tuesday.
Jubril Odekina impressed against the Tottenham Hotspur U21s in the EFL Trophy last Tuesday and kept his place at the weekend, so the 20-year-old is likely to start here.
With no changes expected, U's boss Mark Bonner is likely to name the same side that played at the weekend.
Danny Lloyd returned to the starting lineup for Gillingham at the weekend and is likely to keep his place on Tuesday.
Cambridge United: Mitov; Williams, Masterson, Okedina, Iredale; Tracey, May, Digby, O'Neil, Brophy; Ironside
Gillingham: Cumming; McKenzie, Ehmer, Tucker, Bennett; MacDonald, Lee, Dempsey; Lloyd, Oliver, Sithole
The Cambridge frontman is by far their biggest attacking threat as he leads the U's with five goals and has played all but 38 minutes of the league season.
Much like Ironside, the 29-year-old is his side's leader with three goals and is a workhorse, playing a possible 809 out of 810 minutes.
The last matchup between Cambridge and Gillingham took place in August of 2020 in the second round of the Football League Trophy with the U's running out 2-0 winners.
There will be no live streaming coverage of the match, but live audio commentary is available through the iFollow channel of Cambridge, which is iFollow United. Kick-off is set for 19:45 BST.