Despite what is at stake when it comes to bragging rights, there is a bigger picture for both sides, and that is pushing for a place in the League One play-offs. Wycombe currently sit in eighth, with Oxford United in 10th, both sides are just outside the play-off spots.
In the reverse fixture, the Chairboys came out on top at the Kassam Stadium. After Cameron Brannagan's penalty was brilliantly saved by Wycombe goalkeeper Max Stryjek, the Blues found the winner with a combination of their central defenders on the day.
Wycombe are unbeaten against the Yellows since that final. However, before the final, the Chairboys had not beaten the U's since 2015.
Ainsworth's side have not played for 10 days with their game away to Bristol Rovers being postponed last weekend. Their last game was a 1-0 loss against Sheffield Wednesday
After 10 days without a game, Blues boss Ainsworth could have a fully fit squad coming into the game.
Dominic Gape and Alfie Mawson have both been kept out of the squad as a precaution recently but could well be in the frame to make the squad.
Tjay De Barr has been back in training with his return to the matchday squad imminent.
However, there may be one or two changes with a quick turnover in games. Matt Taylor could be brought in for Yanic Wildschut, with the former having experience in these type of games against the Chairboys.
Oxford went with a back three last time out, with new signing Brandon Fleming playing on the left. They may set up in a similar way after the victory over Ipswich.
Stryjek; Obita, Jacobson, Tafazolli, Grimmer, Wing, Scowen, Mehmeti, Wheeler, McCleary, Vokes (4-2-3-1)
Eastwood; Long, Moore, Brown, Anderson, Fleming, McGuane, Bate, Brannagan, Wildschut, Bodin (3-5-2)
Wycombe - Joe Jacobson
Whilst not having as much game time this season for Wycombe, Joe Jacobson has still played a key part in Wycombe's play-off push so far.
Having played left-back for his entire career, the last two seasons has seen the Welshman operate in more of a centre-half role, giving Ainsworth that extra depth in both positions.
He will be key for Wycombe against Oxford if he does start.
As one of the members of the side which beat the U's in the play-off final, he knows what it takes to beat Karl Robinson's side.
Jacobson scored the winning goal that day for the Chairboys, who will be looking for a similar outcome in this one.
Oxford - Cameron Brannagan
The former Liverpool youngster Cameron Brannagan has played a vital role in putting Oxford back on the right track in their last two games.
Brannagan helped Oxford to wins against Fleetwood Town and Ipswich, with the midfielder scoring the winner against the Tractor Boys last time out.
The midfielder was also part of that game in 2020 and has been at Oxford since 2018, so will have vast experience in these high-tempered games which he'll need to use to Oxford's advantage.
Where is the game being played?
This edition of the M40 Derby will be held in Buckinghamshire at Adams Park.
What time is kickoff?
The game will commence at 19:45 GMT.
How can I watch?
The game will be live on ‘iFollow’ across the world, or on Wycombe Wanderers' ‘WanderersTV’.