This comes after the submission of the final list, following the closure of the transfer window.
This means that the Columbian striker will play no part in Chelsea's last sixteen clash with Paris Saint-Germain or any of their other European ties, should they progress against the French champions.
Falcao not the only absentee
However, it wasn't just Falcao who was omitted from Chelsea's squad.
Former AC Milan goalkeeper Marco Amelia also missed out, despite being named in the Premier League squad.
Similar to the previous five years of his career, the Italian found himself making no appearances for the reigning champions and spending his time sitting on the bench.
The team in full
Here is Chelsea's 25-man 'A list', which had to include eight homegrown players.:
Goalkeepers: Asmir Begovic, Thibaut Courtois and Jamal Blackman.
Defenders: Branislav Ivanovic, Kurt Zouma, Abdul Baba Rahman, Matt Miazga, Gary Cahill, John Terry and Cesar Azpilicueta.
Midfielders: Cesc Fabregas, Oscar, Eden Hazard, John Obi Mikel, Pedro, Nemanja Matic and Willian.
Forwards: Alexandre Pato, Bertrand Traore, Kenedy, Loic Remy and Diego Costa.
Chelsea have also submitted a 'B list' which contains younger players such as Ola Aina, Ruben Loftus-Cheek and goalkeeper Bradley Collins.