Like their visitors, Tranmere are struggling in the league, winless in their last three, but also experienced success in the Football League Trophy and FA Cup.
After a number of changes in midweek, Tranmere manager Micky Mellon will have nearly a full squad to select from this weekend.
Nathaniel Knight-Percival was the only player to start in both the FA Cup win against Crawley Town and the comeback win in midweek. Ross Doohan will again start in goal with Joe Murphy still absent.
Sutton will be without Omar Bugiel, who is away on international duty with Lebanon and Harry Beautyman, out with a shoulder injury. Richie Bennett is suspended while Bradley House is also out.
Craig Eastmond, Louis John and Jon Barden are all in contention to return for the Yellow Army for the trip to Prenton Park.
Tranmere Rovers: Doohan; MacDonald, Knight-Percival, Clarke, Dacres-Cogley; Spearing, O'Connor; Feeney, Watson, McManaman; Nevitt
Sutton United: Bouzanis; Wyatt, Rowe, Goodliffe, Kizzi; Boldewijn, Smith, Milsom, Ajiboye; Olaofe, Wilson
Ones to watch
Jay Spearing (Tranmere Rovers)
The midfielder began his career at Liverpool and played the last four seasons with Blackpool, making 105 appearances for the Tangerines. The 32-year-old was Tranmere's Player of the Year last season after signing for his hometown club.
Donovan Wilson (Sutton United)
He's been Sutton's most productive player in attack, scoring five times including in the Carabao Cup against Cardiff City and had the only goal of the match in the Football League Trophy match against AFC Wimbledon.
In their last matchup, Sutton brought all three points back south thanks to a 32nd-minute goal by Craig Dundas.
Both teams will have streaming coverage on their respective iFollow channels for those outside the UK and Ireland, which are iFollow Tranmere and iFollow the U's. Kick-off time is set for 15:00 GMT.