The South Yorkshire side sit 23rd in table with just 30 points in 39 games, four points adrift of safety.
The Addicks sit 15th in League One, and will want to win the game to steer themselves away from a relegation battle.
Rovers were forced into an early change 13 minutes into their last game, a 0-0 draw against Fleetwood Town, as Kieran Agard had to be replaced by Reo Griffiths up front.
The club have since confirmed that he will be sidelined for the rest of the season with a hamstring injury.
What followed after Agard was replaced was a frustrating day in front of goal for Doncaster, who will hope that they can provide a better attacking threat against Charlton.
Agard, who arrived at the club in January, has yet to score for Gary McSheffrey's side in his four appearances at the club, and losing him to injury will come as a blow to the club.
Winger Jon Taylor has had his return to training delayed due to illness.
McSheffrey says he is not aware of the severity of Taylor's illness.
In more positive news for Rovers, Cameron John has rejoined training after a long spell out with injury.
Jake Forster-Caskey played 66 minutes worth of football for the U23's in midweek as part of his return to fitness.
Charlton were unchanged in their 2-0 win against Burton Albion last time out, and with no news of any injuries, it is quite possible that Johnnie Jackson will name the same side for the third time in a row.
Mitchell, Olowu, Williams, Knoyle, Rowe, Barlow, Smith, Clayton, Griffiths, Martin, Hiwula.
MacGillivray, Lavelle, Clare, Purrington, Matthews, Gilbey, Taylor, Fraser, Dobson, Washington, Stockley.
Rovers come into the game having only won once in their last five and winless in their last four.
It has been a challenging season for the hosts, who have won just eight league games all season.
Doncaster's last win was a 2-0 win against Accrington Stanley on February 22.
Since then in March, Rovers have drawn 2-2 against AFC Wimbledon, lost 4-0 against Cheltenham Town and 0-1 against Gillingham.
Their last game was a goalless draw against Fleetwood Town in what was a huge chance for Doncaster to take points off their relegation rivals.
Should Rovers fail to win on Saturday at the Eco-Power Stadium, it will be the first winless month the club have had since August.
The other issue for McSheffrey and his team is that they have failed to find the back of the net in each of their last three games, which will not help them as they attempt to find a way out of the relegation zone.
The visitors have won their last two games, which ended a long run of poor results for The Addicks.
Their last game, a 2-0 win against Burton Albion followed a 1-0 victory against Gillingham, two vital victories for steering the club away from a relegation battle.
The run before the wins had seen Johnnie Jackson's team lose six of their last seven games.
In truth, after narrowly missing out on the playoffs last term, avoiding a relegation battle will not have been the goal for Charlton at the start of the season.
However, they are at least in a good position to remain in League One next season, and a win on Saturday would go a long way to helping to secure that.
As the club sit 21 points off of the playoffs as the season approaches its conclusion, any hopes of promotion have all but gone for the South-East London club.
Ones to watch
Midfielder Tommy Rowe is the club's top goal scorer for this term depsite not being a striker.
He has hit the back of the net eight times for Rovers, including six goals in Sky Bet League One.
The 33 year old has made 45 appearances for the club this season and is a reliable option for McSheffrey.
He is the only player to score for Doncaster this month, scoring a brace in a 2-2 draw against AFC Wimbledon.
Rowe will be hoping that he can call on his experience to see off Charlton Athletic on Saturday afternoon in a huge chance for Rovers to close the gap to safety.
Charlton's top goal scorer in the league this season is Conor Washington, who has netted ten times for The Addicks this campaign.
Washington scored in the 2-0 win against Burton last time out, and will want to start a scoring streak against a struggling Doncaster side on Saturday afternoon.
The Northern Irishman has scored 21 goals for Charlton in 49 league appearances, meaning the striker can make his 50th league appearance at the club at the Eco-Power Stadium.
The 29 year old will hope he can have a memorable 50th league appearance for the club and help his club to victory against Doncaster.
The head to head between the two sides favours The Addicks, who have won on 10 occasions against Doncaster.
There have been two draws between the two sides in their previous meetings, and Rovers have picked up six victories against Charlton.
In recent years, The Addicks knocked Rovers out of the playoffs, winning 4-3 on penalties after a 4-4 aggregate score in the 2018/19 League One playoff semi-final.
The last meeting between the two sides was an emphatic 4-0 win for Charlton at The Valley back in late October.
Doncaster did not register a single shot on target in a dismal display that day.
Where to watch
Rovers fans from the UK and Ireland can purchase a £2.50 audio match pass via iFollow Rovers, the club's official streaming site.
For fans overseas, there is a £110 season pass to iFollow Rovers which allows fans the opportunity to watch 46 games from their season.
For Charlton fans, supporters from the UK and Ireland are not able to watch the game on CharltonTV due to EFL broadcast restrictions.
Fans outside the UK and Ireland however can purchase a match pass for £10 on their website to watch the game on CharltonTV.
There is also the option to buy a £195 annual pass and a monthly pass for £35 for international supporters to watch the game on.
What the managers have said
Speaking about news that Agard will miss the rest of the season, McSheffrey was disappointed to lose the striker's knowhow at such a vital time in the campaign.
He said: "We put him back in from the start on Saturday looking for his experience.
"It's disappointing. We're gutted for Keiran [Agard] but we support him in whatever way we can to help him get back to full fitness."
McSheffrey will hope that his strikers can step up on Saturday afternoon in his absence.
Speaking after the game against Burton, Jackson said there was hope for his side to continue their good form going forwards.
"I know now with the guys coming back we're going to score goals in games.
"We're scoring goals again and now we're looking more solid so it's really pleasing."
Jackson will be hoping his side can prove his faith in his players is well placed at the Eco-Power Stadium on Saturday.