Star man Harry Kane is yet to score. England will be hoping that they will progress through to the Round of 16 and that their captain can get on the score sheet before then.
Harry Maguire is in contention to start in The Three Lions final group game, he was an unused substitute against Scotland after recovering from an ankle injury.
Jordan Henderson will be looking to get back into England's starting eleven, he has also been battling to be fit after returning from injury. However, good performances from Kalvin Phillips has kept the Liverpool captain on the bench.
After a poor performance in the final third against Scotland, the England manager may well be looking at changing some of his attacking players. England fans are crying out that they want Aston Villa's Jack Grealish to start, will they get their wish on Tuesday?
As for England's opponents, they face no fresh injury issues and look to have a fully fit squad ready for the Wembley encounter. Patrik Schick has recovered from his bloody encounter with Croatia's Dejan Lovren.
Jaroslav Šilhavý will most likely go for a similar team to his sides first two games. If you're leading the group, why change what's not broken?
Czech Republic: Vaclik; Coufal, Kalas, Celustka, Boril; Soucek, Kral; Masopust, Darida, Jankto; Schick
England: Pickford; James, Stones, Mings, Trippier; Phillips, Rice; Grealish, Mount, Sterling; Kane
Player to watch
Czech Republic - Patrik Schick
Schick has scored three goals in his sides two opening Euro 2020 games. The Bayer Leverkusen striker scored from the spot against Croatia after being fouled by former Liverpool defender Dejan Lovren.
He also scored both goals against Scotland as his side won 2-0. His first goal came three minutes before half time as he headed the ball into the bottom corner. However, it was his second goal that grabbed the headlines.
With what could well be the goal of the tournament winner, Schick put Czech Republic 2-0 up seven minutes after the restart. The striker found himself with the ball and with time near the half way line.
After seeing that the Scotland keeper, David Marshall, was off his line, Schick lofted the ball over him leaving his team mates and Czech fans celebrating. Fans around the world soon took to Twitter showing their appreciation of the goal.
England - Jack Grealish
England fans have been pleading Southgate to start the Villa captain. Grealish come on against Scotland in the 63rd minute but didn't get too many opportunities to work with the ball.
For his club in this Premier League season, the 25-year-old has made 16 goal contributions in 26 games. Alongside Ollie Watkins he has been Villa's star man. However that isn't Grealish's stand out stat from this season.
He was the most fouled player in the league this campaign. The skilful and tricky playmaker was fouled 110 times, 22 more times then second placed Wilfried Zaha. This stat goes to show that he's a defenders nightmare and that they struggle against him.
With England's lack of creativity at the moment he is exactly what they need and if played could create problems for Czech Republic.
How to watch
The game will be shown live on ITV, with the game kicking off at 20:00 BST.