Pochettino also confirmed the inclusion of youth players Luke Amos and Kazaiah Sterling.
Much needed rest
It has been clear to many that both Kane and Eriksen have looked physically drained over the past few weeks.
With Kane not quite looking back to full fitness after returning from injury a few weeks ago - this will come as a welcomed break for him.
Speaking to the media ahead of Wednesday's game, Pochettino said: "We are going to rest some players, rotate the starting XI."
However, he added that Dele Alli will start: "Dele is going to play. He missed three Champions League games.
"He needs to play. He is fresh to play."
Edwards still not ready
Marcus Edwards seems to have been on the brink of breaking into the first team for a couple of years now.
The 18-year-old was likened to Lionel Messi in 2016 by his Argentine boss. However, he still feels Edwards is not quite ready.
Pochettino said: "It is up to him to show us and everyone at Tottenham that he deserves it.
"He needs to work hard like different players."
Dele on Spurs' dip
Alli joined the Tottenham boss on Tuesday afternoon as he commented on his sides recent poor Premier League form.
He said: "We’re disappointed with the results in recent games. All clubs have a dip in form. We’ll bounce back and show our character.
"It’s not unusual for a team to have a dip in form. We have a great team, great manager and staff and we’ll bounce back."
The 21-year-old also commented on his own form this season. He added: "I’ve learned a lot from this season, I am my own biggest critic.
"I know if it’s not going well for me but I work hard in every session."