The Foxes embark on their fifth European knockout campaign and will be aiming to rack-up a comprehensive victory ahead of the second-leg at the King Power Stadium next week.
Meanwhile, having already made waves in the Europa League in recent season, Jindřich Trpišovský's Slavia will once again be aiming to claim a memorable scalp.
Route to the knockouts
It was an excellent return to Europe for Leicester City having reached the quarter-finals of the UEFA Champions League back in 2016/17. Matched up with SC Braga, AEK Athens and Zorya Luhansk, the Foxes topped their group, only losing one match to the Ukrainians.
Elsewhere, Slavia came second in Group C behind German giants Bayer Leverkusen. Trpišovský's men wracked up two victories against OGC Nice, whilst also beating Bayer and Hapoel Be'er Sheva to book their place in the Round of 32.
Foxes' boss Brendan Rodgers could be tempted to rotate for the trip to the Czech Republic. Ricardo Pereira is likely to be rotated as he continues his recovery from a serious cruciate ligament injury. He could potentially be replaced by either Christian Fuchs or academy graduate Luke Thomas.
Elsewhere, long-term absentees Dennis Praet, James Justin and Wes Morgan all remain out whilst the game is likely too soon for Timothy Castagne and Wesley Fofana. Ayoze Perez is also ruled out after picking up a knock in the thrilling win against Liverpool.
Much like their opponents, Slavia also have a few injuries to consider. Long-term absentees Yusuf and David Hovorka are still out, whilst midfielder Petr Sevcik is not quite yet ready to make his return to action.
Mick van Buren and Stanisalv Tecl have both suffered slight knocks so are unlikely to feature.
Slavia Prague: Kola; Boril, D. Zima, Kudela, Holes; Masopust, Dorley; A. Sima, Stanciu, Olayinka, Kuchta.
Leicester City: Schmeichel; Thomas, Evans, Soyuncu, Amartey; Ndidi, Tielemans, Maddison; Barnes, Albrighton, Vardy.
Ones to Watch
Young forward Kuchta has been in stunning form since the turn of the year. Since New Year, the 24-year-old has hit nine goals and provided two assists in just six Fortuna Liga appearances having netted twice against FK Pardubice last time out.
He has also been in promising form in the Europa League so far this campaign, scoring twice in a 3-2 win against Ligue 1 OGC Nice, whilst also notching laying on a further two goals in the competition.
Fresh from his electric showing against Liverpool, Leicester wideman Harvey Barnes will be gunning for more goals in Prague. The 23-year-old is undoubtedly in the best form of his Foxes' career having already scored 12 goals in all competitions, his most lethal season in front of goal.
He will look to harness that efficient finishing, as well as his sheer pace, against Slavia on Thursday.
Brendan Rodgers has called on his side to expect a tough game in Prague. Talking ahead of the clash, the Northern-Irishman said: "They have confidence from winning. They are used to winning. They have good pace in the team. The wingers are fast and dynamic.
"Playing in the Premier League, you're playing against different styles, so it won't be anything different to what we've faced before. It's going to be a diffiicult game for us. We've got to be up to the challenge."
How To Watch
Supporters can watch the game on BT Sport ESPN, with coverage set to get underway at 5:30pm. Kick-off is scheduled for 5:55pm.
Otherwise, you can follow every kick with VAVEL's LIVE blog.