A win would take the royals within six points of second place, as long as results go their way.
On the contrary, a win for the owls would prove beneficial in their fight against relegation, with three points potentially taking them to within four points of safety.
Reading could potentially see the return of Yakou Meite to the squad, after a couple of weeks out injured. Michael Morrison is unlikely to appear after coming off midway through their game earlier in the week.
Wednesday may have centre-back Joost Van Aken returning to the squad, as Darren Moore continues to assess his squad.
Rafael; Yiadom, Holmes, Moore, Richards, Laurent, Semedo, Ejaria, Olise, Puscas, Joao.
Wildsmith; Harris, Dunkley, Lees, Borner, Penney, Shaw, Hutchinson, Bannan, Windass, Paterson.
Ones to watch
The Romanian came back in for his first start since November and performed excellently, scoring the only goal in their 1-0 win over Blackburn Rovers on Tuesday night. His hunger was on show, and expect it to be again on Saturday.
The Scotsman has been the owls main creator for a couple of seasons and still is now, boasting the most assists for them this season with four so far.
How to watch
This match will kick off at 15:00 GMT on Saturday, March 7, and is available to watch by purchasing an iFollow pass for £10.