Both sides have started the season poorly, only winning one of the first six games in the league so far this season.
This game poses a massive opportunity for both sides to properly kick start their seasons with a win after such poor starts.
Reading will be without goalkeeper Rafael after breaking his hand, so it is likely Luke Southwood will replace him in the lineup. New signings Scott Dann and Danny Drinkwater may come in for their debuts after missing out on the 3-3 draw with Queens Park Rangers at the weekend due to fitness issues.
Peterborough are likely to have the returns of Nathan Thompson and Jack Taylor, after recovering from injuries. Jonson Clarke-Harrismay also return to the starting lineup after being dropped for Jack Marriott in their 6-2 loss to Sheffield United on Saturday afternoon.
Southwood; Yiadom, Morrison, Moore, Rahman, Laurent, Dele-Bashiru, Halilovic, Swift, Ejaria, Puscas.
Pym; Kent, Knight, Butler, Ward, Coventry, Grant, Burrows, Szmodics, Dembele, Clarke-Harris.
Ones to watch
The midfielder has started off his season like no other, already contributing to eight goals, scoring six and assisting two, including a hat-trick against Queens Park Rangers at the weekend. He is arguably the best player in the league at the moment, so Peterborough will have to keep an eye on him.
The striker netted 31 times for Posh in League One last season, but has so far not had the best of luck, only scoring once. He undoubtedly has the quality and should be good enough for this level, but with Reading's very leaky defence next up for him, he should be looking to score on Tuesday night.
The last time these two sides met was back in 2013, when Peterborough battered Reading 6-0 in the League Cup.
How to watch
The game kicks off at 8 pm on Tuesday September 14, and is available to watch on Sky Sports Red Button.