Jurgen Klopp's side come into the game off the back of a 1-0 victory over Newcastle in the Premier League on Saturday. Whereas, the hosts suffered a 2-1 defeat at Alaves at the weekend.
Klopp has insisted his side are fully prepared for the challenge, he said: "We will be ready, we want to be where we are and we want to go to the final."
Gerard Moreno is a welcome return for Unai Emery's side. The frontman has faced an injury disrupted season, but has still netted 13 times in all competitions.
However, the Yellow Submarine may be without Arnaut Danjuma who has a foot injury, and consequently missed training on Monday.
Roberto Firmino is still unavailable for selection with a foot injury, but otherwise Klopp has a fully fit squad.
Ones to watch
Villarreal - Gerard Moreno
Moreno has netted twice in the competition this campaign, as well as having three assists to his name over six matches.
His potential place back in the starting line up could provide some fresh challenges for LFC's backline.
Liverpool - Luis Diaz
Diaz has been a revelation since his arrival at LFC, and looked a threat throughout in the first leg of this fixture.
The Colombian netted in the Reds previous away match in this competition and will be confident that he can make a similar impact on Tuesday night.
Villarreal: Rulli, Foyth, Albiol, Torres, Estupinan, Chukwueze, Capoue, Parejo, Trigueros, Lo Celso, Moreno.
Liverpool: Alisson, Alexander-Arnold, Konate, Van Dijk, Robertson, Fabinho, Henderson, Thiago, Salah, Mane, Diaz.
Emery's side will have to take a more front foot approach to make up for the two goal deficit, which could play into LFC's hand with the pace of Salah, Mane and Diaz.
The Reds have won all five of their away matches in the competition this campaign, and that run should continue here.
Prediction - Villarreal 1-3 Liverpool
How to watch
The match will be available to watch in the UK on BT Sport, with kick-off commencing at 8PM.