The Reds still sit at the top of the table with rivals Manchester City close on their heels.
Chris Wilder's Sheffield United also progressed through in the FA Cup at the weekend, they beat Plymouth Argyle 2-1 with The Blades dominating most parts of the game.
Wilder's men are currently at the bottom of the league table in 20th, with 19 games played Sheffield United have only managed to pick up five points this season.
Marcus Rashford is available for Manchester United on Wednesday night. There were some doubts around whether the forward would be fit after he went straight down the tunnel after being substituted against Liverpool. Ole Gunnar Solskjær said that Rashford "tweaked his knee a little bit but should be fine."
Victor Lindelof and Eric Bailly have both been deemed fit but are also being carefully managed.
John Egan will be missing for The Blades through suspension, whilst Jack O'Connell is still recovering from a knee injury. Sander Berge picked up an injury in the reverse fixture of this game and remains unavailable.
Ben Osborn is expected to remain on the side line and there are still question marks around the fitness of both Oli McBurnie and Lys Mousset.
Manchester United: De Gea; Wan-Bissaka, Maguire, Lindelof, Shaw; Matic, Fred; Greenwood, Fernandes, Martial; Cavani
Sheffield United: Ramsdale; Basham, Egan, Ampadu; Bogle, Lundstram, Norwood, Fleck, Stevens; McGoldrick, Sharp
Ones to watch
For Manchester United, Bruno Fernandes will pose a threat as he always does. The attacking midfielder scored the winner against Liverpool at the weekend after coming off of the bench. The Portuguese international seems to be going from strength to strength at United and he doesn't seem to be slowing down any time soon.
Mason Greenwood was also world class at the weekend, the young forward scored United's first and assisted their second. United will be wanting to get the 19-year-old's confidence up again, like it was last season and who better to do it against than the team at the bottom of the table?
And for The Blades club captain Billy Sharp has scored in both of Sheffield United’s last two wins and will provide a physical battle for both centre-backs if he is involved on Wednesday. So will David McGoldrick, who scored twice when Sheffield United and The Reds met at Bramall Lane in December. The striker is the team’s leading goal scorer this season, having found the net five times in the Premier League and once in the Carabao Cup.
Head to head
Sheffield United have not beaten The Red Devils in the Premier League since 15 August, 1992. And with the form that the away side are in I can't see that changing any time soon.
The past five games between the two sides have resulted in four wins for Manchester United and one draw. The most recent game was in December when Manchester United won 3-2. Marcus Rashford scored twice and Anthony Martial added a third with David McGoldrick scoring early and late for Wilder's side.
In the last home meeting between the sides Martial scored his first hat-trick for Manchester United when they won 3-0.
The stats weigh heavily in favour of Ole Gunnar Solskjær's side. However, in a season where the unlikely has happened no one can be sure that this will be a walk in the park for the home side.
How to watch
The game at Old Trafford is set to kick off at 20:15 (BST) and will be broadcasted live on TV on BT Sport.
Ole Gunnar Solskjær when talking about United's form, he said: “I’ve said it so many times, home or away, it doesn’t really matter.
"It’s just the surface of the pitch and there are no fans, no crowd.
"You don’t have that extra man that we usually have, which we can’t wait to get back again.
"The ground staff have done a good job, so we’re happy with with the surface.
"Our form has come through hard work, through the coaching, through the work the players have done.
"We knew the beginning was going to be hard for us and it just fell that we had a few difficult home games to start with and circumstances in those games were hard for us to overcome.
"Now the form is good, the mood is good, the confidence is good, the squad is fit and healthy.
"We are in a good moment.
"Of course, you have to enjoy it, but it’s focus on the next one.
"That’s the only thing that matters.”
Chris Wilder when talking about the game on Wednesday evening, he said: “It’s a huge challenge for us and the players but we’ve nothing to lose.
"We’re not expected to get anything, so go out there and give it a go.
“The odds and opinions will all be in Manchester United’s favour.
"We can’t do anything about that.
"If I was a neutral I would expect it to be a comfortable night for Man Utd.
“We have got to make it as uncomfortable as possible for them.”
He continued by saying: “I can’t affect the form Man Utd are in or the players we are up against.
"All we can do is control our team, our attitude and the way we go about it.
“Without being defeatist, if it’s their night and they play to the top of their ability it is going to be a really tough night for us, but we hope that is not the case and we flip that around.
"We will be doing all we can to try to affect that.”