The competition has been a welcome distraction from a poor Premier League campaign so far, however they looked to have turned a corner after wins over Manchester City and West Ham before losing to newly-promoted Nottingham Forest last weekend.
Last time's match-winner Matip misses out, his recovery from a calf injury is taking longer than expected and Thiago Alcantara is still recovering from the ear infection that kept him out of the loss to Forest at the weekend.
However Darwin Nunez and Ibrahima Konate are available after returning to training after their hamstring and muscle injuries respectively.
Brazilian goalkeeper Alisson Becker is expected to make his 200th Reds appearance.
Kudus is expected to get the nod over Brian Brobbey, despite the latter scoring a brace in last weekend's 4-1 win at RKC Waalwijk.
Midfielder Kenneth Taylor is a doubt with a muscle strain and youngsters Ahmetcan Kaplan, Mohamed Ihattaren and Devyne Rensch are also ruled out.
Liverpool: Alisson; Alexander-Arnold, Konate, Van Dijk, Robertson; Fabinho, Henderson, Elliott; Nunez, Firmino, Salah
Ajax: Pasveer; Sanchez, Bassey, Timber, Blind; Berghuis, Alvarez, Klaassen; Bergwijn, Kudus, Tadic
Liverpool- Mohamed Salah
He then scored the winner in the win against Man City.
The burden on Liverpool's attacking players is now even heavier with the long-term losses of Diaz and Jota; if anyone can step-up in their absence, it is Salah.
Ajax- Edson Alvarez
He was also linked to a reunion with former manager Erik ten Hag at Manchester United, due to his ability to play in the six or as a central defender.
He will be crucial to breaking up the play and setting up counter attacks for the hosts.
Where is the game being played?
The game is being played at the Johan Cruijff Arena in Amsterdam.
When is kick-off?
Kick-off is at 20:00 BST on Wednesday 26th October.
How can I watch?
The match is live on BT Sport 2, with coverage starting at 19:00 BST.