Swindon Town will be attempting to progress to the second round of the competition for the first time since 2014, whereas Cambridge will be looking to be in the draw for the next round for three consecutive years in a row.
Cambridge are expected to make a 'number of changes', according to manager Mark Bonner.
Midfielder Jensen Weir, on loan from Brighton & Hove Albion, trained on Monday morning and will be available for the fixture.
Winger Jack Lancaster and defender Leon Davies have joined up with the rest of the squad to train following injuries, however the game may come too soon for them.
Swindon will be looking to rotate their squad as much as possible, with Ben Garner's side not having many minutes of pre-season under their belt due to the their late start.
Aston Villa loanee Kaine Kesler will be assessed for the game after coming off injured against Scunthorpe, however the head coach has confirmed there is 'nothing major there'.
Defender Akin Odimayo and midfielder Jordan Lyden will be available after missing the season opener due to having COVID-19.
Ones to watch
The forward will be looking to add another goal to his tally after netting a penalty to salvage the U's a point on Saturday.
The home sides number nine will be looking to cause problems against a defence that may be a slightly easier challenge than their third tier opposition on Saturday.
The Swindon backline will seek to keep the physical striker as quiet as possible in this cup fixture.
A new addition to the Robins in the last few days, the midfield maestro's arrival came as a shock to the fanbase as not many could believe a player of his calibre had dropped to the bottom tier.
As he did not sign the dotted line in time to be eligible for Swindon's League Two opener, it is expected to see Louis Reed start on Tuesday night.
Bonner and his Cambridge side will be keeping a keen eye on the former Peterborough United orchestrator as he will look to control the tempo of the game from the midfield.
What the managers have said
Cambridge manager Mark Bonner spoke about the importance of managing the squad after a difficult pre-season, he said:
“The number one aim is to progress in the competition, but at the same time, we need to make sure that with the number of pre-season games that we’ve had, some boys need minutes and games, and I think that’s important to try and keep the squad level.
“We’ve got a busy week ahead after this, with two away games and then a home game against Burton, and we need real freshness going into that, but we also need a whole squad fit and ready to go and contribute in the league games.
"They played well actually at Scunthorpe and got a good result, and for them I’m sure the buzz was great off the back of a turbulent and tortuous summer, and really quite a large part of last year.
“I think having worked at a club that’s gone through those really challenging times here, we know what that’s like, so you can have some empathy towards what that’s like as a player, as a member of staff, as a supporter, when your club goes through those challenging times."
(Quotes via cambridge-news.co.uk)
Swindon head coach Ben Garner:
"We will make a few team changes but this is an opportunity and it gives players an opportunity to fight for a shirt."
"We are underdogs for tomorrow given the circumstances we have had, Cambridge had a fantastic season last season but that won't faze us.
"We look forward to the challenge of going there."
Garner confirmed that no major injuries had been picked up from the weekend, he said:
"We have a couple of players to assess today but haven't picked up any long-term injuries from Saturday."
(Quotes via totalswindon.com)
How to watch
Kick-off on Tuesday 10 August is 19:45 BST. Those living in the UK and Ireland are unavailable to watch the game on iFollow or an equivalent club streaming service, although those in other regions can purchase a match pass and watch the action live.
The game has not been chosen for Sky Sports coverage either, whilst tickets are available to both sets of supporters who would like to attend in person.