On 34 points, Brighton sit seven points and two places above the relegation zone while the Blades sit rock-bottom of the Premier League with just 14 points to their name.
The Seagulls picked up their first-ever point at Chelsea when they drew 0-0 at Chelsea last Tuesday while Sheffield United were officially condemned to the EFL Championship after a 1-0 defeat at Wolverhampton Wanderers.
Sander Berge is the only known player to be definitely ruled out for Sheffield United with a thigh injury. Billy Sharp also has a thigh injury, but he, Jack O'Connell (knee), Oli McBurnie (foot), Chris Basham (calf) and Jack Robinson (match fitness) are all still a question mark to play on Saturday.
Brighton look to be without Florin Andone and Percy Tau as both are doubtful with hamstring injuries while Ben White serves his one-match ban after picking up a red card at Stamford Bridge.
Sheffield United: Ramsdale; Ampadu, Egan, Bryan; Baldock, Lundstram. Norwood, Osborn, Stevens; McGoldrick, Brewster
Brighton & Hove Albion: Sanchez; Webster, Dunk, Veltman; Burn, Bissouma, Lallana, Gross; Welbeck, Trossard; Maupay
Ones to watch
David McGoldrick has accounted for six of Sheffield United's 17 goals this season, which amounts to 35%. While that seems to be an impressive statistic, consider only Wolves and Fulham have lower top scorers in the top flight than the Blades.
Brighton goalkeeper Robert Sanchez made his first start in the fixture in December and has gone on to keep eight clean sheets in his 18 starts in the Premier League, making manager Graham Potter's decision to drop the now-departed Mathew Ryan for the Spaniard look like a stroke of genius.
On December 20th, the two sides played out a 1-1 draw at the AMEX Stadium. John Lundstram picked up a red card in the 40th minute lead and despite being down to 10 men, Jayden Bogle gave Sheffield United a 63rd-minute lead.
It looked like the Blades would pick up three valuable points until Danny Welbeck finally made good use of Brighton's man advantage, scoring an equalizer three minutes from time.
How to watch
The match will be shown on Sky Sports Premier League and Main Event with coverage beginning at 7:45pm. The game will kick off at 8pm.