The U's currently sit just three points behind sixth placed Portsmouth, albeit having played one game more, and two wins in a row have set their challenge back on track.
Meanwhile, the Gills' aspirations of making the play-offs have drastically decreased following a defeat to Blackpool and draw to Shrewsbury Town. It leaves them six points off having played more games than almost everyone around them in the table.
Rob Hall and Alex Gorrin are also back in training, which leaves John Mousinho and Marcus McGuane as the only other absentees.
Gillingham manager Steve Evans will have the benefit of no fresh injury concerns going into the match.
Oxford United (4-3-3): Stevens - Long, Moore, Atkinson, Ruffels - Henry, Brannagan, Sykes - Lee, Taylor, Shodipo.
Gillingham (4-4-2): Bonham - Jackson, Cundy, Tucker, Ogilvie - Graham, Dempsey, O'Keefe, MacDonald - Oliver, Akinde.
Oxford go into this fixture having won three out of their last five matches. Their last two games saw them produce ten different scorers in the impressive 6-0 away thrashing of Crewe Alexandra and 4-1 victory over Shrewsbury. This has given the U's momentum going into their last five games which might propel them to a play-off finish.
On the other hand Gillingham have only won once in their last five. A heavy away defeat to Blackpool plus a 0-0 draw against Shrewsbury has left them with only the thinnest of chances of making the play-offs. They are six points off in 10th position at this moment in time.
Ones to watch
James Henry may be the one to watch for Oxford. Whilst the attacking prowess of the entire team has been demonstrated in their last two matches, Henry has contributed two goals and three assists in his last five matches.
He's proved himself to be a driving creative force from the midfield and a huge benefit to the team as a whole.
Vadaine Oliver will be the one to watch for Gillingham. The striker notched nine goals in nine games in March, winning him the Sky Bet League One player of the month in the process.
Whilst his aerial threat is an obvious point for the Oxford defence to be wary of, he's more than capable of providing quality from anywhere in the opposition half.
The two sides last met in the reverse fixture earlier this season, where Gillingham ran out 3-1 winners back in October. Goals from Vadaine Oliver and Jacob Mellis put the Gills 2-0 ahead at half time, and a third effort from Jordan Graham was enough to restore the two goal advantage following Dan Agyei's strike for Oxford right at the beginning of the second half.
In the overall head to head record Gillingham also come out on top, having won 19 of the fixtures compared to Oxford's 14. There have also been 15 encounters where the points have been shared.
How to watch
The game has not been selected for TV coverage in the UK, but an iFollow pass can be purchased for £10 via either club's website to stream the game live.
Kick-off is scheduled for 17:30 BST.