Seleção das Quinas opened their campaign with a victory over Ghana, where a flurry of late goals meant they secured maximum points in their first game.
La Celeste hit the post twice during the stalemate through Diego Godin's header from a corner and Federico Valverde's late long-range effort.
Midfielder-turned-centre-back Danilo Pereira will miss Portugal's second group match after suffering three fractured ribs in their opening win.
However, they looked in greater control of the tie when William Carvalho was introduced early on in the second half.
The Real Betis midfielder could be handed a start alongside goalscorer Leao who gave them a different dynamic in attack with his raw pace.
Barcelona defender Ronald Araujo will once again miss out after undergoing thigh surgery before the start of the tournament.
It is unsure when the centre-back is likely to return, but Jose Gimenez and Godin will fill in during the meantime.
Alonso has a decision to make, as veteran striker Luis Suarez looked like a shadow of his former self in their opening game.
(4-3-3) Costa, Guerreiro, Dias, Pepe, Cancelo, Carvalho, Neves, Fernandes, Silva, Ronaldo, Felix
(4-3-3) Rochet, Caceres, Godin, Gimenez, Olivera, Valverde, Vecino, Bentancur, Pellistri, Cavani, Nunez
The attacking midfielder put himself at the top of the assists charts for the tournament with two in their opening victory.
But the Manchester United midfielder will hope for better movement ahead of him as they face a more stubborn Uruguay defence.
At times the Portuguese looked devoid of any attacking fluidity and this should be something Santos looks at ahead of their second match.
Fernandes will be key to any sort of momentum they have in the final third.
Liverpool's summer signing has five Premier League goals to his name so far and will be looking to make his mark on the world stage.
The forward joined the Reds from Benfica for a club-record fee and has impressed so far.
Despite largely starting in a central position for his club, Nunez is utilised on the wings for his national team.
With Cavani tipped to start in the central position there should be no excuse for Alonso's side not to score for the second-consecutive group game.
Where is the game being played?
The game will be played at Lusail Iconic Stadium, Lusail, Qatar.
What time is kick-off?
Kick-off is set for 7pm (GMT UK) on Monday, November 28.
How can I watch it?
In the UK, the game will be broadcast live on ITV, with audio commentary available on both BBC 5Live and talkSPORT.