It was his first match in charge of the club and Conte likely wanted to get a sense of what his strongest starting XI could be. But that will not be the case this time around.
So, let's take a look at who VAVEL think he will play and why it should be exciting for Spurs fans.
The second-choice goalkeeper, Pierluigi Gollini, should get a run-out against Mura on Thursday as he looks to make a case for a permanent Tottenham transfer.
The Ireland right-wing-back has the most experience in this back three formation than anyone in the Tottenham squad, having played it rather successfully at Wolves.
Off the back of a couple of fine performances for his national side during the break, Matt Doherty could be a very realistic contender for the starting spot in the future.
He came on in the second half against Leeds and looked very impressive, particularly in the air and defending on the break.
Joe Rodon -
Joe Rodon has been rather harshly starved of football so far this season, despite still being a regular for Wales. He is another one who could really suit a back three and we are excited to see him get a run-out against Mura.
It has long since been argued whether Japhet Tanganga is a full-back or centre-back. Fortunately, a back three provides a hybrid position that could just be perfect for him. It will be interesting to see him get a run of games on the right or left side.
It was so good to see Ryan Sessegnon back in action again at the weekend and it's fair to say he looked bright. The left-wing-back even scored a goal, albeit offside in the build-up.
Much like Tanganga, Sessegnon is another who was stuck between positions, left-back and left-wing, but could profit from the emergence of a left-wing-back role.
With Hojbjerg stepping up with goals as well as midfield muscle, could we see the academy graduate rise to the occasion?
Conte has already made it clear, Tanguy Ndombele will have to work hard and get his fitness up if he is to play a regular part this season.
It will be interesting to see what kind of performance he gives after being unused against Leeds United. Will he sink or look to prove a point?
Conte often makes the most of one or two number ten slots, so Dele has an opportunity, on paper at least, to earn a place under the new boss.
I have a feeling he may really rise to the occasion after a few years away from his best.
Steven Bergwijn was not called up for his country during the international break, before eventually being drafted in as a late injury replacement.
The Spurs man then proved to be the hero by scoring and assisting in a 2-0 win to send the Netherlands to the World Cup. If that isn't the perfect springboard to take into club football, I don't know what is.
We are all dying for this boy to get opportunities in front of goal. Dane Scarlett has been handed minutes so far this season, but has often cut an isolated or frustrated figure up top.
With a more attacking wing-back formation under Conte, it will be interesting to see whether the youngster can shine.